Varian Star Chromatography Workstation Manually
A semi-micro method has been developed using preparative thin-layer chromatography (TLC) to separate acylglycerols for the subsequent analysis by gas chromatography–mass spectrometry (GC–MS). Monoacylglycerols (MAGs) were formed from butter oil by fungal degradation with Penicillium roquefortii. Total lipids were extracted with hexane–2-propanol (3:2, v/v) and separated on silica gel preparative TLC plates with fluorescence indicator (Merck). The plates were developed in hexane–diethyl ether–formic acid (80:20:2, v/v). Lipid bands were detected under UV light or with iodine vapour, removed and then extracted with hexane–2-propanol (3:2, v/v). The MAG band ( R F 0.03) was silylated into trimethylsilyl (TMS) ethers.
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Structures and composition of MAG-TMS ethers were analysed by GC and GC–MS. Formation of characteristic ions for the identification of sn-1(3)- and sn-2-MAG isomers was discussed.
The method is simple, inexpensive and powerful for the separation and analysis of relatively small amounts of MAGs (0.2–5.0 mg) formed from fungal degradation. Previous article in issue. Next article in issue.
| Public (NASDAQ: VARI) | |
| Industry | Scientific instruments |
|---|---|
| Fate | Acquired by Agilent Technologies |
| Founded | 1999 |
| Headquarters | Palo Alto, California |
Key people | Allen J. Lauer, Chairman Garry Rogerson, President & CEO |
| Products | Scientific instruments |
| 3,700 | |
| Website | www.varianinc.com |
Varian, Inc. was one of the largest manufacturers of scientific instruments for the scientific industry. They had offerings over a broad range of chemical analysis equipment, with a particular focus on Information Rich Detection[clarification needed] and Vacuum technology. Varian was spun off from Varian Associates in 1999 and was purchased by Agilent Technologies in May 2010 for $1.5 billion, or $52 per share.[1]
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Varian Inc. had its corporate headquarters in Palo Alto, California, and offices in Australia, the Benelux countries, Brazil, Canada, China, Germany, France, Italy, Japan, Korea, Russia, Sweden, Taiwan, the United Kingdom, and the United States.
Manufacturing Plants[edit]
Varian Inc. contained the following manufacturing plants:
Lake Forest, California USA
Palo Alto, California USA
Walnut Creek, California USA
Lexington, Massachusetts, USA
Cary, North Carolina, USA
Mulgrave, Victoria Australia (the largest Varian Inc manufacturing and R&D plant)
Middelburg, Netherlands
Grenoble, France
Yarnton, United Kingdom
Church Stretton, Shropshire, United Kingdom (polymer laboratories)
Turin, Italy
Products and services[edit]
Chromatography, including gas chromatography, GC/MS, high performance liquid chromatography, and LC/MS.
Spectroscopy, including UV-Vis, near infrared, and FT-IR
Inductively coupled plasma, including mass spectroscopic and atomic emission spectroscopic detection.
mass spectrometry, including FTMS
Nuclear magnetic resonance
Linear accelerator
Magnetic resonance imaging
X-ray Crystallography
Information rich detection
Drugs of Abuse Testing
Dissolution Testing
Vacuum Pumps
Leak Detection
Turbo Pumping Systems
Vacuum Measurement
Valves and components
History[edit]
In October 1967, Techtron Pty Ltd merged with Varian Associates. Techtron is a manufacturer of Atomic Absorption Spectrometers and Spectral lamps.
In 1982 Varian transferred the Cary UV-Visible product line to Australia.
In 2002 Varian bought Ansys Technologies, Inc., a California-based manufacturer of In-Vitro Medical Devices.
In 2004 Varian Inc bought Magnex Scientific, an Oxford-based manufacturer of high-field magnets.
In 2005 Varian bought Polymer Laboratories, a speciality polymer analysis and manufacturing company.
In 2006 Varian bought Ion Spec, an FTMS (Fourier Transform Mass Spectrometry) manufacturing company.
In 2007, Varian, Inc. bought Analogix, Inc., a company specializing in flash chromatography.
In 2008, Varian bought Oxford Diffraction, a British company specializing in X-ray diffraction equipment.[2]
On 27 July 2009 Agilent Technologies announced it would buy Varian Inc, for $1.5 Billion.
On 14 October 2014 Agilent made the strategic decision to close its NMR business. Agilent entered the NMR business in 2010, with the acquisition of Varian.[3]
See also[edit]
References[edit]
- ^''Agilent reaches deal to buy Varian for $1.5 billion' (Press release)'. Reuters. 2009-07-27. Retrieved 2009-07-27.
- ^''Varian, Inc. Acquires Oxford Diffraction, Ltd.' (Press release)'. Retrieved 2008-07-03.
- ^''Agilent Technologies to Close Nuclear Magnetic Resonance Business' (Press release)'.